Hydrogen phosphate. hydrogenphosphate. Hydrogen phosphate ion. UNII-33UE6C4909. tetraoxidophosphate(.2-) More Molecular Weight: 95.979 g/mol. Dates: Modify . Liming. Create . Liming. Hydrogenphosphate is a phosphate ion that is the …
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Hydrogen Bond Donor Count: 1: Computed by Cactvs 3.4.6.11 (PubChem release 2019.06.18) Hydrogen Bond Acceptor Count: 3: Computed by Cactvs 3.4.6.11 (PubChem release 2019.06.18) Rotatable Bond Count: 0: Computed by Cactvs 3.4.6.11 (PubChem release 2019.06.18) Exact Mass: 79.966331 g/mol: Computed by PubChem 2.1 (PubChem release 2019.06.18
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phosphate buffer - University of Nebraska–Lincoln
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Other buffers perform minor roles than the carbonic-acid-bicarbonate buffer in regulating the pH of the blood. The phosphate buffer consists of H 2 PO 4-in equilibrium with HPO 4 2-and H +. The pK for the phosphate buffer is 6.8, which allows this buffer to function within its optimal buffering range at physiological pH.
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Normally, phosphate buffer at neutral pH is prepared by mixing dihydrogenphosphate and hydrogendiphosphate solutions having the same molarity. As long as sodium cations or chloride anions are not critical to your protein and your chromatographic method, the working pH could also be adjusted very slightly by the small addition of NaOH (to dihydrogenphosphate) or HCl (to hydrogendiphosphate).
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